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However, the determination of neural and glial lineages from embryonic stem cells has not been accomplished. We have used a combination of immunocytochemistry and mRNA expression studies to show that undifferentiated mouse ES cells can be induced to generate large numbers of neural progenitors. These progenitors express markers characteristic of the central nervous system (CNS) at a relatively early stage of induction and some neuronal markers at later stages. The expression of some of these markers is regulated at the transcriptional level. These results demonstrate that in the presence of morphogens, mouse ES cells can be induced to express the early CNS markers Nestin and Pax6, and to a lesser extent, Otx2, which is present in several neural lineages. 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